Biochemistry · Enzymes and Clinical Enzymology

Factors affecting enzyme activity

5 min read

Quick Orientation

Section

Definition

Objective
Understand the core idea
Keywords
Definition

Effect of Enzyme and Substrate Concentration

Objective
Learn the main facts and relationships
Keywords
Enzyme concentration, Substrate concentration, Km

Substrate Kinetics: Km and Vmax

Objective
Learn the main facts and relationships
Keywords
10⁻⁵ to 10⁻² mol, Lineweaver-Burk double reciprocal plot

Effect of Temperature

Objective
Learn the main facts and relationships
Keywords
Temperature, 35°C to 40°C, Q10 (temperature coefficient)

Effect of pH

Objective
Learn the main facts and relationships
Keywords
pH, neutral pH (6 to 8), acidic pH (4 to 6)

Clinical Correlation

Objective
Connect the mechanism to clinical findings
Keywords
Serum enzyme diagnostic assay, Food preservation, Induced hypothermia surgery

Definition