Biochemistry · Enzymes and Clinical Enzymology

Enzyme kinetics: Km, Vmax and Michaelis-Menten concept

3 min read

Quick Orientation

Section

Definition

Objective
Understand the core idea
Keywords
Km (Michaelis-Menten constant, moles/L, 10⁻⁵ and 10⁻² moles

Formation of the Enzyme-Substrate Complex

Objective
Learn the main facts and relationships
Keywords
enzyme-substrate complex (ES), Km = (k2 + k3) / k1

Michaelis-Menten Equation

Objective
Learn the main facts and relationships
Keywords
Michaelis-Menten equation, Step 1, Step 2

Interpretation of Km

Objective
Learn the main facts and relationships
Keywords
Low Km, high Km, not dependent on the concentration of enzyme

Enzyme Reactions with Two or More Substrates

Objective
Follow the sequence and key control points
Keywords
two or more substrates, Michaelis-Menten kinetics

Clinical Correlation

Objective
Connect the mechanism to clinical findings
Keywords
Clinical Correlation

Definition