Quick Orientation
Section
Definition
- Objective
- Understand the core idea
- Keywords
- Polymerase chain reaction (PCR), in vitro, Kary Mullis
Principle and Stages of PCR
- Objective
- Learn the main facts and relationships
- Keywords
- denaturation, renaturation, synthesis
Sources of DNA Polymerase
- Objective
- Learn the main facts and relationships
- Keywords
- Taq DNA polymerase
Applications of PCR
- Objective
- Learn the main facts and relationships
- Keywords
- clinical diagnosis, sex determination of embryos, DNA sequencing
Clinical Correlation
- Objective
- Connect the mechanism to clinical findings
- Keywords
- Prenatal diagnosis of inherited disease, sickle-cell anemia, beta-thalassemia
Definition
Polymerase chain reaction (PCR) is an in vitro, cell-free technique that synthesizes multiple identical copies (billions) of a specified DNA.